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KMID : 0369819920220010063
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Jorunal of Korean Pharmaceutical Sciences
1992 Volume.22 No. 1 p.63 ~ p.68
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Quantitation of Flurbiprofen in Isopropyl Myristate by High Performance Liquid Chromatography
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김현/Kim H
지상철/Chi SC
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Abstract
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An HPLC procedure with UV detection has been developed for the quantitation of flurbiprofen released into isopropyl myristate used as the receptor phase in an in vitro membraneless drug diffusion cell. The drug and the internal standard (oxaprozin) were extracted from isopropyl myristate with a mixture of dimethylsulfoxide:methanol:water (2:1:1) and quantitated using a reverse phase C18 column. The chromatograms were completely free from interfering peaks, and the relative retention times of flurbiprofen and the internal standard were 4.9 and 6.8 min, respectively. Calibration plots were linear over the concentration range of 1?200μg/ml of flurbiprofen with correlation coefficients, all higher than 0.99. The mean intra-day precision and accuracy among three replicate sets of the assay in a day were 4.26 and 4.52%, respectively, whereas the mean inter-day precision and accuracy were 3.35 and 3.64%, respectively. The mean recovery of the drug was 92.5% over the calibration range. The method was simple, reliable and accurate for the quantitation of flurbiprofen in unpurified isopropyl myristate.
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KEYWORD
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flurbiprofen, isopropyl myristate, HPLC, assay validation
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